Journal: Human Molecular Genetics

Article Title: Impact of Fgf10 deficiency on pulmonary vasculature formation in a mouse model of bronchopulmonary dysplasia

doi: 10.1093/hmg/ddy439

Figure Lengend Snippet: Antibodies for immunohistochemistry

Article Snippet: Phospho MYL6b , BosterBio (A14059) , 1:600 (mouse).

Techniques:

Journal: Scientific Reports

Article Title: Skeletal myosin binding protein-C isoforms regulate thin filament activity in a Ca 2+ -dependent manner

doi: 10.1038/s41598-018-21053-1

Figure Lengend Snippet: Regulation of muscle contraction by MyBP-C isoforms was determined using recombinant proteins representing the N-terminal region of slow-skeletal, fast-skeletal, and cardiac isoforms of MyBP-C. ( A ) Schematic diagram of full-length slow-skeletal, fast-skeletal, and cardiac MyBP-C isoforms (ssMyBP-C, fsMyBP-C, and cMyBP-C, respectively). Domains are numbered C0 to C10 from the N-terminus, and proline-alanine region (PA) is common to all isoforms. Circles denote immunoglobulin-like domains, and pentagons represent fibronectin type 3 domains. Yellow lines identify known phosphorylation sites; red lines indicate cardiac-specific insert in C5 domain of cMyBP-C. ( B ) SDS-PAGE demonstrates the relative size and purity of each MyBP-C recombinant protein, encompassing the N-terminal region, up to and including the C2 domain. ( C – F ) Permeabilized ventricular rat papillary muscles were left untreated (control, white column) or were incubated with 10 µM ssC1C2 (red), fsC1C2 (blue), and C0C2 (black) and allowed to undergo muscle contraction analysis by the Force-ATPase assay. ( C ) Relative force-pCa curves and ( D ) quantification of pCa 50 values from the relative force-pCa curves demonstrate significant increases of fsC1C2 and C0C2 in Ca 2+ -sensitivity of force development. ( E ) Rate of tension redevelopment ( k tr ) was determined using a rapid release and restretch maneuver at maximal Ca 2+ levels (pCa 4.5) and submaximal Ca 2+ levels (pCa 6). Top trace is of ( F ) at pCa 6, and k tr was significantly enhanced by fsC1C2 and C0C2. Graphs represented as mean ± SEM, *p < 0.05 vs . controls, **p < 0.01 vs . controls, # p < 0.05 vs . ssC1C2/fsC1C2, n = 7–9 animals.

Article Snippet: Detection of protein was determined by using antibodies for c-Myc, ssMyBP-C, fsMyBP-C, and cMyBP-C (Sigma C3956, ProSci 6679, ProSci 5651, and Santa Cruz sc-137180, respectively) (Supplemental Figure ).

Techniques: Recombinant, SDS Page, Incubation, ATPase Assay

Journal: Scientific Reports

Article Title: Skeletal myosin binding protein-C isoforms regulate thin filament activity in a Ca 2+ -dependent manner

doi: 10.1038/s41598-018-21053-1

Figure Lengend Snippet: Greater activation of thin filament results in prolonged diastole. ( A ) To determine how each isoform regulates dynamic contraction, full-length MyBP-C adult rat ventricular myocytes (ARVM) were infected with adenoviral constructs (MOI 1000) overexpressing full-length, cMyc-tagged slow-skeletal, fast-skeletal, or cardiac MyBP-C, followed by 48 h culture. ( B ) Immunofluorescence (IF) imaging demonstrates localization of adenoviral-mediated expression of MyBP-C isoforms (green) within the sarcomere, as delineated by α-actinin (red). ( C , D ) Unloaded shortening was measured by changes in sarcomere length (SL) during dynamic contraction and relaxation (ARVM paced at 1 Hz, 20 V, 2 ms). ( C ) Relaxation kinetics was measured by time to % baseline, how fast the cell returns to 10, 50, and 90% of resting SL, and ( D ) relaxation constant tau, a logarithmic fit of the relaxation curve. ( E ) Changes in relaxation kinetics were evident by combined traces, and in silico simulations demonstrate that greater thin filament activation can contribute to relaxation kinetics. Graphs represented as mean ± SEM, *p < 0.05 vs . uninfected controls, # p < 0.05 vs . cMyBP-C.

Article Snippet: Detection of protein was determined by using antibodies for c-Myc, ssMyBP-C, fsMyBP-C, and cMyBP-C (Sigma C3956, ProSci 6679, ProSci 5651, and Santa Cruz sc-137180, respectively) (Supplemental Figure ).

Techniques: Activation Assay, Infection, Construct, Immunofluorescence, Imaging, Expressing, In Silico

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 4. Effect of yak milk on immunohistochemistry of kidney in mice. (A) Images of mouse kidney tissue cross sections immunostained for TRPV5 and calbindin-D28k (scale bar = 100 μm). (B) Relative mean density of TRPV5 in mouse kidney. (C) Relative mean density of calbindin-D28k in mouse kidney. Mean density = (integrated optical density [IOD] sum)/area. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with different lowercase letters (a–d) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Immunohistochemistry

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 5. Effects of yak milk on the expression of calcium transporters TRPV5 and calbindin-D28k in mouse kidney. (A) Western blot showing the expression of TRPV5 and calbindin-D28k in mouse kidney. (B) Quantification of TRPV5 expression from the Western blots. (C) Quantification of calbindin-D28k expression from the Western blots. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with dif- ferent lowercase letters (a–c) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Expressing, Western Blot

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 6. Effects of yak milk on the expression of calcium transporters TRPV5 and calbindin-D28k mRNA in mouse kidney. (A) Relative expression of TRPV5 mRNA in mouse kidney. (B) Relative expression of calbindin-D28k mRNA in mouse kidney. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with different lowercase letters (a–d) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Expressing